Method for producing apoprotein
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United States of America Patent
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Issued Date -
Feb 11, 2010
app pub date -
Dec 28, 2005
filing date -
Jan 5, 2005
priority date (Note) -
Abandoned
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Abstract
A method and an apparatus for producing an apoprotein with which the apoprotein can be efficiently produced from a protein associated with cofactors such as metal ions. The method includes the step of adding an acid to a solution which contains a holoprotein, which is associated with a cofactor which is to be released under acidic conditions, and concentrating the solution with an ultrafiltration membrane. Accordingly, an apoprotein, which has released its cofactor, is produced, and the released cofactor is removed through the membrane along with the acid without reassociating with the apoprotein.
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