System and Method for Consensus-Calling with Per-Base Quality Values for Sample Assemblies
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United States of America Patent
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app pub date -
Jul 29, 2008
filing date -
Oct 25, 2001
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Abandoned
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Abstract
The present teachings disclose a method for evaluation of a polynucleotide sequence using a consensus-based analysis approach. The sequence analysis method utilizes quality values for a plurality of aligned sequence fragments to identify consensus basecalls and calculate associated consensus quality values. The disclosed method is applicable to resolution of single nucleotide polymorphisms, mixed-based sequences, heterozygous allelic variants, and heterogeneous polynucleotide samples.
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