Kinase and phosphatase assays
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United States of America Patent
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Nov 29, 2011
Grant Date -
Sep 23, 2010
app pub date -
Mar 22, 2010
filing date -
Jul 29, 2003
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Abstract
Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.
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