Polyanion for improved nucleic acid amplification
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United States of America Patent
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Mar 22, 2011
Grant Date -
Oct 7, 2010
app pub date -
Aug 25, 2009
filing date -
Sep 9, 2008
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Abstract
The present invention is directed to a novel chemical compound comprising the structure [Xx-(CH2)m-phosphate-Yy]n, characterized in that 3≦m≦6, 30≦n≦60, each x and y is independently from each other 0 or 1, each X and Y is independently from each other any photometrically measurable entity; provided that the terminal X can also be an —OH group or a phosphate group, and further provided that the terminal Y can also be an —OH group. Such a compound can be used as a suitable hot start additive for PCR based amplification of nucleic acids.
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