Enzymatic synthesis of optically pure beta-hydroxy carboxylic acids and their derivatives
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United States of America Patent
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Issued Date -
Oct 21, 2010
app pub date -
Mar 21, 2007
filing date -
Mar 21, 2007
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Abstract
The present disclosure is related to systems, compositions, and methods for producing a β-hydroxy carboxylic acid and/or a β-hydroxy carboxylic amide in enantiomeric excess (e.g., enantiomerically pure). In some specific example embodiments, a method may include contacting a substrate and/or intermediate with a cyanide, a ketoreductase, a nitrilase, and/or a nitrile hydratase. Systems, compositions, and methods, in some embodiments, may produce a β-hydroxy carboxylic acid and/or a β-hydroxy carboxylic amide in enantiomeric excess under convenient conditions (e.g., pH 5-9 and temperatures under 50° C.).
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