Manufacturing method of separating and purifying neoagarooligosaccharides having degrees of polymerization from 2 to 22
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United States of America Patent
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Issued Date -
Dec 23, 2010
app pub date -
Jun 18, 2009
filing date -
Jun 18, 2009
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Abandoned
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Abstract
MAEF108, respectively, into an agar polysaccharide extract solution, and obtaining a neoagarooligosaccharides solution after a hydrolysis on the algal polysaccharide (such as agar) is performed; performing a separation to the neoagarooligosaccharides solution by a ultrafiltration (UF) system to obtain a neoagarooligosaccharide eluent; performing a separation to the neoagarooligosaccharide eluent by a semi-preparative high-performance liquid chromatography system equipped with a molecule size exclusion chromatography function to obtain the neoagarooligosaccharides having the degrees of polymerization from 2 to 22; and using a fraction collector to collect the neoagarooligosaccharides, and obtaining purified single neoagarooligosaccharide products with different molecular masses after a freeze-drying process is performed to the neoagarooligosaccharides.
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