METHODS FOR PRODUCING MODIFIED GLYCOPROTEINS
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United States of America Patent
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Issued Date -
Jan 27, 2011
app pub date -
Oct 11, 2010
filing date -
Jun 28, 2000
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Abstract
or other structures along human glycosylation pathways. This is achieved using a combination of engineering and/or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.
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