Method of Scanning a Sample Plate Surface Mask in an Area Adjacent to a Conductive Area Using Matrix-Assisted Laser Desorption and Ionization Mass Spectrometry
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United States of America Patent
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app pub date -
Nov 9, 2010
filing date -
Apr 30, 2003
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Abstract
A method of scanning a sample plate surface mask in an area adjacent to a conductive area using mass spectrometry is disclosed. The method comprises the steps of providing a sample plate including a mask applied with a rough surface to the electrically conductive surface to produce a sample site comprising a central portion formed from the electrically conductive surface and a marginal portion of the mask, preparing an analyte comprising mixing a biomolecule with an organic solvent, an aqueous solution, and a matrix selected from the group of α-cyano-4-hydroxycinnamic acid and 3,5-dimethoxy-4-hydroxycinnamic acid; applying the analyte to the sample site; forming at least one crystal of the analyte in an area on the mask adjacent to the conductive area, and scanning the area on the mask adjacent to the conductive area with a laser beam.
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