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Abstract

The present invention provides, among other things, engineered microorganisms and methods that allow efficient conversion of soy carbohydrates to industrial chemicals by fermentation. In some embodiments, the invention provides microbial cells engineered to have increased efficiency in utilizing a soy carbon source (e.g., soy molasses, soy meal, and/or soy hulls) as compared to a parent cell. In some embodiments, microbial cells are engineered to have altered (e.g., increased) expression or activity of one or more carbohydrate modifying enzymes (e.g., glycosidases). In some embodiments, microbial cells are engineered to have altered localization of carbohydrate modifying enzymes (e.g., glycosidases). In some embodiments, engineered microbial cells provided herein are used to produce industrial chemicals (e.g., surfactin) using soy components as primary or sole carbon sources.

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First Claim