METHOD OF PRODUCING FUSED PROTEIN
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United States of America Patent
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Issued Date -
Mar 17, 2011
app pub date -
Aug 21, 2007
filing date -
Aug 21, 2006
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Abstract
Described herein is a polynucleotide encoding a fusion protein comprising two or more polypeptide domains and a polypeptide linker joining the domains, wherein the sequence of the polynucleotide encoding the polypeptide linker is selected such that when the mRNA transcribed from the polynucleotide is translated in a host cell transfected with the polynucleotide, the translation rate of the mRNA region encoding the polypeptide linker is slower than the translation rate of the mRNA region encoding the polypeptide domain immediately upstream thereof. Also provided are a vector transfected with the polynucleotide of the present invention so that the polynucleotide can be expressed in the host cell; a host cell transformed by that vector; and a process for producing a fusion protein comprising culturing the host cell, and recovering the fusion protein thus produced.
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