Method of differentiating stem cells
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United States of America Patent
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Feb 9, 2021
Grant Date -
Apr 14, 2011
app pub date -
Oct 13, 2010
filing date -
Oct 13, 2009
priority date (Note) -
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Abstract
The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture medium having an osmolality less than 340 mOsm/kg. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media for use in inducing the three germ layers.
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