Method for isolating nucleic acids
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United States of America Patent
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Apr 14, 2015
Grant Date -
Apr 28, 2011
app pub date -
Oct 15, 2010
filing date -
Oct 22, 2009
priority date (Note) -
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Abstract
A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids.
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