Polypeptides Modified by Protein Trans-Splicing Technology
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United States of America Patent
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Issued Date -
May 26, 2011
app pub date -
Dec 12, 2008
filing date -
Dec 13, 2007
priority date (Note) -
Abandoned
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Abstract
The present invention relates to a method of preparing modified polypeptides, by linking a target polypeptide to a carrier molecule that is designed to bear one or more water-soluble polymer molecules, via protein trans-splicing. The polymer molecules can be attached to the carrier molecule either before or after ligation to the target polypeptide. Novel protein trans-splicing elements (known as “split inteins”) and trans-splicing partners are also provided.
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