Protein having fructosyl valyl histidine oxidase activity, modified protein, and use of the protein or the modified protein
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United States of America Patent
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Mar 31, 2015
Grant Date -
Aug 11, 2011
app pub date -
Oct 6, 2009
filing date -
Oct 10, 2008
priority date (Note) -
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Abstract
The present invention provides: a protein having a fructosyl amino acid oxidase activity which protein is useful for measurement of a glycosylated protein (particularly, glycosylated hemoglobin); a modified protein thereof; and use of the protein or the modified protein. The protein of the present invention is, for example, a fructosyl valyl histidine oxidase derived from Phaeosphaeria nodorum, the fructosyl valyl histidine oxidase having excellent thermal stability and substrate specificity and also having a small Km value to fructosyl valyl histidine. This allows a glycosylated protein measuring reagent to be stored in a long time and measurement accuracy of the glycosylated protein measuring reagent to be improved.
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