EVALUATION OF 24S-HYDROXYCHOLESTEROL IN HAIR FOR METABOLIC BIOMARKER OF ALZHEIMER'S DISEASE
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United States of America Patent
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Issued Date -
Aug 25, 2011
app pub date -
Feb 25, 2010
filing date -
Feb 25, 2010
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Abandoned
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Abstract
Disclosed is a method for detection of 24S-hydroxycholesterol in hair. More specifically, a trace amount of hair is hydrolyzed under basic condition and metabolites including 24S-hydroxycholesterol are purified and extracted by solid-phase extraction (SPE). Then, after derivatization of the resulting extract with trimethylsilyl (TMS), 24S-hydroxycholesterol is detected by gas chromatography-mass spectrometry (GC-MS).
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