Electrotransformation of Gram-Positive, Anaerobic, Thermophilic Bacteria
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United States of America Patent
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Issued Date -
Nov 17, 2011
app pub date -
Oct 13, 2009
filing date -
Oct 30, 2008
priority date (Note) -
Abandoned
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Abstract
The present invention relates to methods for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation and Gram-positive, anaerobic, thermophilic bacteria transformed by the disclosed methods. The methods employ voltage pulsing schemes that decrease arcing such that increased transformation efficiency and cell viability is observed. The present invention is further directed to a method for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation using recovery/selection temperatures to effect increased transformation efficiency in difficult to transform bacteria.
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