METHOD FOR THE REDUCTION OF REPETITIVE SEQUENCES IN ADAPTER-LIGATED RESTRICTION FRAGMENTS
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United States of America Patent
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Issued Date -
Nov 24, 2011
app pub date -
Nov 25, 2009
filing date -
Dec 4, 2008
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Abandoned
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Abstract
The present invention relates to a method for the reduction of repetitive sequences (or the improvement in low-copy sequences) in DNA samples by a combination of restriction endonuclease treatment, followed by adapter ligation, renaturation kinetics-based fractionation, optionally coupled with duplex sequence nucleases and further restriction endonuclease treatment, followed by adapter ligation. The low-copy enriched fractions can be used in further DNA analysis.
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