Method and Apparatus for Addressable Flow Cells in Single Molecule Sequencing
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United States of America Patent
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Issued Date -
Dec 22, 2011
app pub date -
Mar 16, 2011
filing date -
Mar 16, 2010
priority date (Note) -
Abandoned
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Abstract
A method of sequencing a plurality of template nucleotide sequences includes immobilizing the plurality of template nucleotide sequences on a substrate. A first subset of the plurality of template nucleotide sequences is immobilized in a first field of view and a second subset of the plurality of template nucleotide sequences is immobilized in a second field of view. The first and second subsets are hybridized to a caged primer. The caged primer includes a caging group. The method further includes lysing the caging group from the caged primer in the first field of view and observing the first field of view to detect sequencing of the first subset of the plurality of template nucleotide sequences.
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