USE OF DOUBLE STRANDED RNA TO INCREASE THE EFFICIENCY OF TARGETED GENE ALTERATION IN PLANT PROTOPLASTS
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United States of America Patent
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Issued Date -
Dec 22, 2011
app pub date -
Dec 22, 2009
filing date -
Dec 22, 2008
priority date (Note) -
Abandoned
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Abstract
Method for targeted gene alteration in protoplasts of plant cells comprising the steps of transiently transfecting the protoplasts with a dsRNA that preferably targets plant MMR mRNA; and a mutagenic nucleobase. The transfection may be simultaneously or subsequently and the gene can be any gene functional in the mismatch repair system.
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