Media Solutions and Methods for Cryopreservation and Thawing of In Vitro Fertilization Specimens
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United States of America Patent
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Issued Date -
Mar 15, 2012
app pub date -
Sep 22, 2011
filing date -
May 28, 2008
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
A medium solution which will increase the growth, survival and ultimately the live birth rate of oocytes and embryos which have been or will be subjected to cryopreservation. The solution contains varied amounts of glucose, pyruvates, amino acids, vitamins K5 and C, antioxidants, fatty acids to supply the specimens with the chemical ingredients and uptake requirements required to recover and prosper during and after the cryopreservation process. The solution supplies nutrients to the specimens that will replenish depletion and damage to the specimens and their mitochondria, spindles and structural features, such as cell walls. One formulation addresses the additional requirements of frozen specimens as opposed to the current media solutions and methods which treat the unfrozen specimens the same as the frozen specimens when recovering them from cryopreservation.
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