DETECTION OF DNA HYDROXYMETHYLATION
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United States of America Patent
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Issued Date -
Mar 15, 2012
app pub date -
Dec 8, 2010
filing date -
Sep 9, 2010
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Abstract
Reagents and methods for analysis of DNA hydroxymethylation are provided. Methods comprise modification of hydroxymethylated cytosine residues with a bulky moiety to protect hydroxymethylated positions from cleavage with a DNA endonuclease. For example, methods may comprise contacting DNA with a glucosyltransferase to glucosylate hydroxymethylated DNA positions and digesting the DNA with a DNA endonuclease to cleave DNA in positions lacking hydroxymethylation. Reagents and kits for hydroxymethylated DNA analysis are also provided.
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