T-DNA/PROTEIN NANO-COMPLEXES FOR PLANT TRANSFORMATION
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United States of America Patent
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Issued Date -
Mar 22, 2012
app pub date -
Jun 2, 2011
filing date -
Jun 2, 2010
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Abandoned
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Abstract
A DNA/protein nano-complex for transfecting eukaryotic cells, wherein the nano-complex comprises an expressible nucleotide molecule complexed with a VirD2 protein molecule, and a RecA protein molecule complexed with the nucleotide molecule/VirD2 protein molecule complex. The nucleotide molecule/VirD2 protein molecule/RecA protein molecule complexed may be further complexed to a cell penetrating peptide. The DNA/protein nano-complex can be used for delivering the expressible nucleotide molecule to target plant cells, in particular, monocot plant cells exemplified by wheat cells, corn cells, triticale cells, barley cells, and rye cells.
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