Method for determining crystallization parameters and apparatus for use with the same
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United States of America Patent
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Nov 6, 2012
Grant Date -
Mar 29, 2012
app pub date -
Nov 30, 2011
filing date -
May 10, 2005
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Abstract
The present disclosure provides a method to allow a user to pre-screen numerous crystallization conditions in the crystallization space to identify those conditions with the highest probability of yielding crystals and high quality diffracting crystals. In one embodiment, the dilute solution thermodynamic virial coefficient, termed B, is used to aid in the determination crystallization conditions that increase the probability of producing crystals for the crystallant of interest. The present disclosure also provide methods for predicting solution conditions that generate beneficial solubility and/or stability conditions for a polypeptide of interest using the B parameter. Devices for use in the described methods are also described.
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