METHOD FOR AN IN VITRO MOLECULAR EVOLUTION OF ANTIBODY FUNCTION
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United States of America Patent
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app pub date -
May 16, 2011
filing date -
Apr 5, 2000
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Abandoned
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Abstract
The present invention relates to a method for in vivo molecular evolution of antibody function. According to the present invention, a nucleic acid encoding a CDR that is normally contained in a framework (the “original framework”), which differs from a selected master framework, is amplified from an immunoglobulin gene and is inserted into a nucleic acid encoding the selected master framework. The invention further provides an antibody library, such as a phage display library, and methods of making the same.
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