METHOD OF SCREENING FOR INSULIN SECRETION-POTENTIATING AGENTS
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United States of America Patent
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Issued Date -
Apr 5, 2012
app pub date -
Apr 15, 2010
filing date -
Apr 17, 2009
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Abstract
Disclosed are a novel method of screening for insulin secretion-potentiating agents as well as means for performing such screening. The means include a DNA encoding fluorescent-labeled Epac2 comprising two different DNAs encoding two different fluorescent proteins which emit fluorescent light with wavelength differing from each other and a DNA encoding Epac2 which are fused together in-frame, and the cells transformed with the DNA. Also disclosed is a method of screening insulin secretion-potentiating agents comprising bringing a candidate compound into contact with cells transformed with the said DNA, and detecting whether the compound binds to Epac2.
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