US Patent Application No: 2012/0100,530

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ENZYME MUTANT

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Abstract

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The invention relates to constructs comprising a nucleic acid binding protein and a surface. At least one native accessible cysteine residue is removed from the binding protein. The binding protein is attached to the surface via one or more accessible cysteine residues. The removal of other accessible cysteine residues from the protein allows control attachment to the surface. The constructs can be used to generate transmembrane pores having a nucleic acid binding protein attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect each of its component nucleotides by stochastic sensing.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
OXFORD NANOPORE TECHNOLOGIES LIMITEDKIDLINGTON, GB3

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Jayasinghe, Lakmal - 6 6
Knaggs, Michael - 7 2
McKeown, Brian Oxford, GB 11 4
Milton, John Nr. Saffron Walden, GB 45 234
Moysey, Ruth - 4 0
White, James Lincoln, NE 67 6825298

Cited Art Landscape

Patent Info (Count) # Cites Year
 
OXFORD NANOPORE TECHNOLOGIES LIMITED (1)
* 2011/0229,877 ENZYME-PORE CONSTRUCTS 2 2009
* Cited By Examiner

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