Methods and Oligonucleotide Designs for Insertion of Multiple Adaptors Employing Selective Methylation
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United States of America Patent
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Issued Date -
May 17, 2012
app pub date -
Feb 4, 2011
filing date -
Nov 5, 2007
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Abandoned
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Abstract
Aspects described and claimed herein provide methods to insert multiple DNA adaptors into a population of circular target DNAs at defined positions and orientations with respect to one another. The resulting multi-adaptor constructs are then used in massively-parallel nucleic acid sequencing techniques.
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