PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION COMPRISING THE USE OF AN ALPHA-1,2-MANNOSIDASE
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United States of America Patent
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Issued Date -
May 31, 2012
app pub date -
Jul 27, 2011
filing date -
Jul 30, 2010
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Abandoned
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Abstract
A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of one or more α-1,2-mannosidases from Coccidiodes immitis, Coccidiodes posadasii, Penicillium citrinum, Magnaporthe grisea, Aspergillus saitoi, Aspergillus oryzae, and Chaetomiun globosum or a catalytically active fragment of said α-1,2-mannosidase.
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