HIGH THROUGHPUT DETECTION OF MOLECULAR MARKERS BASED ON AFLP AND HIGH THROUGH-PUT SEQUENCING

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United States of America Patent

SERIAL NO

13364799

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Abstract

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The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular marker.

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KEYGENE N VNot Provided

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hogers, Rene Cornelis Josephus Ede, NL 15 1013
Van, Eijk Michael Josephus Theresia Herpen, NL 73 5112

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