METHOD OF ESTIMATING SEPSIS RISK IN AN INDIVIDUAL WITH INFECTION
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United States of America Patent
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Issued Date -
Jun 14, 2012
app pub date -
Oct 9, 2009
filing date -
Oct 9, 2008
priority date (Note) -
Abandoned
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Abstract
A method of estimating sepsis risk in an individual with infection comprises a step of assaying a biological sample from the individual for an IL-2 or IL-7 mRNA value, and correlating the mRNA value with sepsis risk. The IL-2 and IL-7 mRNA values are quantified by absolute quantification of mRNA copy number, wherein the copy numbers are normalised to a house keeping gene and corrected against a calibration curve for serial dilutions of the IL-2 and IL-7 cDNA. The method generally involves a step of assaying a biological sample from the individual for IL-2 and/or IL-7 mRNA values, optionally in combination with mRNA values for other cytokines, and correlating a sum or difference of the values with sepsis risk using a regression analysis curve against outcome.
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