TEMPERATURE-RESPONSIVE CELL CULTURE SUBSTRATE ON WHICH A STRAIGHT-CHAIN TEMPERATURE-RESPONSIVE POLYMER IS IMMOBILIZED, AND MANUFACTURING METHOD THEREFOR
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United States of America Patent
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Issued Date -
Jun 21, 2012
app pub date -
Aug 27, 2010
filing date -
Aug 27, 2009
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Abandoned
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Abstract
Provided is a temperature-responsive cell culture substrate. A non-crosslinked temperature-responsive polymer having a molecular weight between 10,000 and 150,000 is immobilized on the substrate surface with a density of 0.02 to 0.3 molecular chain per square nanometer. Using the provided temperature-responsive cell culture substrate, cells obtained from various tissues can be efficiently cultured. This culturing method makes it possible to efficiently peel off a cell sheet by just changing the temperature, without causing damage.
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