Methods for preparing vesicles and formulations produced therefrom
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United States of America Patent
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Mar 6, 2018
Grant Date -
Jul 12, 2012
app pub date -
Jul 6, 2010
filing date -
Jul 6, 2009
priority date (Note) -
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Abstract
The present disclosure provides methods for preparing vesicles. In general, these methods include steps of providing a lyophilized lipid product and rehydrating the lyophilized lipid product with an aqueous solution comprising an antigen such that antigen-containing vesicles are formed. The lyophilized lipid product is prepared by dissolving vesicle-forming lipids in a polar-protic water-miscible organic solvent to produce a lipid solution and then lyophilizing the lipid solution. The present disclosure also provides antigen-containing vesicle formulations prepared using these methods. The present disclosure also provides kits that include a lyophilized lipid product in a first container and an aqueous solution comprising an antigen in a second container.
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