Reagents and methods for classifying leukocytes
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United States of America Patent
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Nov 6, 2012
Grant Date -
Jul 19, 2012
app pub date -
Mar 23, 2012
filing date -
Dec 24, 2007
priority date (Note) -
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Abstract
A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.
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