DRAIN DOWN AND RE-FEED OF MICROCARRIER BIOREACTOR
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United States of America Patent
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Issued Date -
Jul 26, 2012
app pub date -
Jan 20, 2012
filing date -
Jul 23, 2009
priority date (Note) -
Abandoned
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Abstract
Disclosed is a method of increasing product yield per culture in a population of bound product-secreting cells in a bioreactor, the method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration; re-feeding the bound population of product-secreting cells by adding an amount of a fresh culture medium sufficient to increase the volume of the culture medium to approximately the original volume of the culture medium; agitating the culture medium under sufficient conditions and for a sufficient time period to allow the bound population of product-secreting cells to grow and to release a second-secreted product concentration into the culture medium; and harvesting product.
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