BASE-BY-BASE MUTATION SCREENING
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United States of America Patent
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Issued Date -
Sep 27, 2012
app pub date -
Oct 29, 2010
filing date -
Nov 4, 2009
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
Aspects of the present invention are drawn to screening assays for isolating polynucleotides having a sequence variation or mutation. Embodiments of the screening assays include generating a population of polynucleotide duplexes having 5′ overhang regions on one strand of the duplex (the “template” or “bottom strand”) followed by isolating polynucleotide duplexes from the mixture that have one or more mismatched base at the 3′ end of the other strand of the duplex (the “test” or “top” strand).
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