TRANSFORMED HUMAN PLURIPOTENT STEM CELLS AND ASSOCIATED METHODS
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United States of America Patent
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Issued Date -
Oct 4, 2012
app pub date -
Sep 1, 2010
filing date -
Sep 1, 2009
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Abstract
The present disclosure provides transformed human pluripotent stem cell (t-hPSC). t-hPSCs are not dependent on Oct4 for renewal and survival, however exhibit a sensitivity to reduced levels of the transcription factor Nanog. Also provided are methods of culturing cells for use in a cell-based screening assay comprising placing one or more transformed human pluripotent stem cells into a receptacle and culturing said stem cells in the receptacle to form a monolayer of stem cells without cell overlap. Methods of screening compounds using t-hPSCs are also described.
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