Capturing sequences adjacent to type IIs restriction sites for genomic library mapping
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United States of America Patent
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Jun 14, 2011
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Sep 16, 1994
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Abstract
The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
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