METHODS OF PURIFYING VIRUSES USING GEL PERMEATION CHROMATOGRAPHY
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United States of America Patent
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Issued Date -
Nov 1, 2012
app pub date -
Apr 27, 2012
filing date -
Apr 29, 2011
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Abstract
Provided herein are elution buffers and methods for purifying viruses using gel permeation chromatography. The methods are useful, for example, in increasing recovery of a virus from a gel permeation chromatography column. The buffers for use in the methods include at least one excipient selected from histidine or sucrose, a divalent cation, a non-ionic detergent, and a phosphate buffered saline.
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