NOVEL REPORTER-TAGGED RECOMBINANT MEMBRANE PROTEINS WITH TRANSMEMBRANE LINKERS
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United States of America Patent
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Issued Date -
Jan 10, 2013
app pub date -
Jun 6, 2012
filing date -
Jun 6, 2011
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Abandoned
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Abstract
Recombinant protein constructs are described that comprises a membrane protein whose N- or C-terminus in the native state is recombinantly linked through a membrane-spanning linker polypeptide to a reporter polypeptide. The reporter polypeptide may be a fluorogen activating protein capable of binding a fluorogen to detect the location and relative abundance of the membrane protein, and more specifically to detect protein trafficking to the cell surface using a cell impermeant fluorogen probe.
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