METHODS AND TRANSPOSON NUCLEIC ACIDS FOR GENERATING A DNA LIBRARY
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United States of America Patent
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app pub date -
Jul 9, 2012
filing date -
Jul 11, 2011
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Abstract
A method for the generation of DNA fragmentation library based on a transposition reaction in the presence of a transposon end with an engineered cleaveage site providing facilitated downstream handling of the produced DNA fragments, e.g., in the generation of sequencing templates. Transposon nucleic acids comprising a transposon end sequence and an engineered cleaveage site located in the sequence, e.g., in Mu transposon end sequence, are disclosed.
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