RNA- AND DNA-COPYING ENZYMES
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United States of America Patent
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Issued Date -
Jan 24, 2013
app pub date -
Feb 4, 2010
filing date -
Feb 4, 2009
priority date (Note) -
Abandoned
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Abstract
The present invention is directed to DNA polymerase fusion proteins with increased processivity and nucleic acid affinity. The invention includes a fusion protein comprising a nucleic acid-binding domain fused to a polymerase domain. The nucleic acid binding domain contains at least one nucleic acid binding motif, such as a DNA-binding motif or an RNA-binding motif. The nucleic acid binding domain preferably embodies an oligonucleotide/oligosaccharide binding (OB) fold, among other conformations. The invention further includes methods of synthesizing nucleic acids using the fusion proteins described herein.
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