METHODS FOR OPTIMIZING OPTICAL MAPPING CONDITIONS
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United States of America Patent
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Issued Date -
Jan 31, 2013
app pub date -
Jul 26, 2011
filing date -
Jul 26, 2011
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Abandoned
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Abstract
The invention generally relates to methods and apparatuses for optimizing conditions for optical mapping. In certain embodiments, methods of the invention involve providing a substrate including a gradient of silanes in a first direction, introducing to the substrate, a gradient of enzyme activity in a second direction, contacting a plurality of enzymes and a plurality of nucleic acids to the substrate, and analyzing enzymatic activity and interaction of the nucleic acids with the substrate, thereby determining the optimal conditions for optical mapping of the nucleic acid.
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