METHOD FOR ISOLATING RNA FROM WHOLE BLOOD SAMPLES
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United States of America Patent
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Issued Date -
Feb 14, 2013
app pub date -
Aug 10, 2012
filing date -
Aug 11, 2011
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Abstract
The present invention relates to a method for isolating RNA from a whole blood sample, comprising the steps bringing the sample into contact with an aqueous lysis solution containing at least one lysis substance in a concentration of 1.5 mol/1 to 7 mol/1 and at least one detergent, simultaneously or subsequently adding a proteinase, in particular proteinase K, then incubating the solution for at least partial enzymatic digestion and lysis of the sample so that a lysate is obtained, and isolating the RNA from the lysate.
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