Region of Interest Extraction and Normalization Methods
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United States of America Patent
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Issued Date -
Feb 28, 2013
app pub date -
Feb 14, 2011
filing date -
Feb 22, 2010
priority date (Note) -
Abandoned
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Abstract
Aspects of the present invention are drawn to methods and compositions for the genetic analysis of regions of interest from one or more starting polynucleotide sample, e.g., a multiplexed polynucleotide sample. In certain aspects, a polynucleotide sample comprising one or more region of interest (ROI) is subjected to independent amplification reactions for specific sub-regions within the ROI(s). The amount/concentration of the product from each sub-region amplification reaction is determined followed by producing a normalized sample based on the determined amount/concentration that is suitable for further analyses (e.g., sequencing).
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