Self-Assembled Bead-Based Multiplexed Assay For Antigen-Specific Antibodies
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United States of America Patent
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Issued Date -
Feb 28, 2013
app pub date -
Aug 24, 2012
filing date -
Aug 25, 2011
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
A flexible method for making multiplexed assays for antigen-specific antibody responses is disclosed herein. The method of the present invention comprises incubation of a substrate or a set of beads coated with a dockerin or a cohesin protein with a cohesin or dockerin-antigen fusion proteins to attach the fusion protein essentially irreversibly by non-covalent interaction. The present invention enables the use of one dockerin- or cohesin-coated bead for multiple sets of cohesin- or dockerin-antigens, obviating the need to directly chemically couple new antigens to new beads.
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International Classification(s)
- C07H:SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTI....
- C12Q:MEASURING OR TESTING PROCESSES INVOLVING ENZYMES O....
- B82Y:SPECIFIC USES OR APPLICATIONS OF NANO-STRUCTURES; ....
- C12N:MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF
- G01N:INVESTIGATING OR ANALYSING MATERIALS BY DETERMININ....
- C40B:COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL ....
- C07K:PEPTIDES
- B01D:SEPARATION
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