PRIMER SET FOR PCR, PEACTION LIQUID FOR PCR, AND METHOD FOR DETECTING FOOD POISONING BACTERIA
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United States of America Patent
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Issued Date -
Mar 7, 2013
app pub date -
Apr 11, 2011
filing date -
Apr 14, 2010
priority date (Note) -
Abandoned
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Abstract
Nucleic acid of two or more types of food poisoning bacteria among Bacillus cereus, Campylobacter, Escherichia coli, Listeria, Salmonella, Staphylococcus aureus, and Vibrio parahaemolyticus is specifically amplified to simultaneously and specifically detect the food poisoning bacteria. More specifically, nucleic acid of food poisoning bacteria is amplified by PCR using a PCR primer set that includes two or more primer sets selected from a primer set for amplifying DNA of Bacillus cereus (SEQ ID NO: 1 and SEQ ID NO: 2), a primer set for amplifying DNA of Bacillus cereus (SEQ ID NO: 3 and SEQ ID NO: 4), a primer set for amplifying DNA of Campylobacter (SEQ ID NO: 5 and SEQ ID NO: 6), a primer set for amplifying DNA of Escherichia coli (SEQ ID NO: 7 and SEQ ID NO: 8), a primer set for amplifying DNA of Listeria (SEQ ID NO: 9 and SEQ ID NO: 10), a primer set for amplifying DNA of Salmonella (SEQ ID NO: 11 and SEQ ID NO: 12), a primer set for amplifying DNA of Staphylococcus aureus (SEQ ID NO: 13 and SEQ ID NO: 14), and a primer set for amplifying DNA of Vibrio parahaemolyticus (SEQ ID NO: 15 and SEQ ID NO: 16).
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