DETECTION OF DNA METHYLATION
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United States of America Patent
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Mar 2, 2011
filing date -
Mar 3, 2010
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Abandoned
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Abstract
In a first aspect, the invention concerns a method for detecting or quantifying DNA methylation at a locus. In one embodiment, a methylation-sensitive endonuclease is formulated together with a polymerase enzyme in an appropriate reaction mixture such that amplification of DNA occurs in a methylation specific manor. Quantitative DNA amplification at selected loci can be used to determine the level of methylation. Kits and reagents for performing such methods are also provided.
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