RAPID METHOD FOR IDENTIFYING DRUG-RESISTANT GENE USING ARTIFICIAL CHROMOSOME OF PLASMODIUM, AND METHOD FOR PREPARING RECOMBINANT PLASMODIUM
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United States of America Patent
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Issued Date -
Mar 28, 2013
app pub date -
Mar 25, 2011
filing date -
Mar 26, 2010
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Abandoned
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Abstract
The present invention relates to a rapid method for identifying a drug-resistant gene using an artificial chromosome of a protozoa. Specifically, it relates to a method for screening for a drug-resistant gene, which involves preparing a recombinant Plasmodium using an artificial chromosome of a Plasmodium, either inoculating the recombinant Plasmodium into a non-human mammal or using the in vitro culture system of the Plasmodium at the blood stage in the red blood cells, and using the survival of the recombinant Plasmodium in the presence of a drug as an index. The present invention also relates to a method for preparing a recombinant Plasmodium by directly introducing an exogenous gene into a Plasmodium at a high efficiency.
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