Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 10378051
APP PUB NO 20130085073A1
SERIAL NO

13627134

Stats

ATTORNEY / AGENT: (SPONSORED)

Importance

Loading Importance Indicators... loading....

Abstract

See full text

A reaction mixture including (a) a nucleic acid having a primer hybridized to a template, (b) nucleotide analogs, wherein the nucleotide analogs have a blocking moiety; (c) a polymerase that is capable of forming an extended primer by adding the nucleotide analogs to the primer, and (d) a deblocking agent that is capable of removing the blocking moiety from the extended primer. Also provided is a method of synthesizing a polynucleotide including sequentially adding a plurality of the different nucleotides analogs to the nucleic acid via several reaction cycles in the reaction mixture, wherein each reaction cycle includes (i) the polymerase adding a nucleotide analog to the nucleic acid to form a transient nucleic acid species comprising a blocking moiety, and (ii) the deblocking agent modifying the transient nucleic acid species to remove the blocking moiety.

Loading the Abstract Image... loading....

First Claim

See full text

Family

Loading Family data... loading....

Patent Owner(s)

Patent OwnerAddress
ILLUMINA CAMBRIDGE LIMITEDCAMBRIDGE CB21 6DF

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cressina, Elena Nr Saffron Walden, GB 14 52
Jackson, Rosamond Nr Saffron Walden, GB 10 117
Liu, Xiaohai Nr Saffron Walden, GB 106 4424
Meuleman, Wouter Nr Saffron Walden, GB 14 218
Smith, Geoffrey Paul Nr Saffron Walden, GB 58 2693

Cited Art Landscape

Load Citation

Patent Citation Ranking

Forward Cite Landscape

Load Citation

Maintenance Fees

Fee Large entity fee small entity fee micro entity fee due date
7.5 Year Payment $3600.00 $1800.00 $900.00 Feb 13, 2027
11.5 Year Payment $7400.00 $3700.00 $1850.00 Feb 13, 2031
Fee Large entity fee small entity fee micro entity fee
Surcharge - 7.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge - 11.5 year - Late payment within 6 months $160.00 $80.00 $40.00
Surcharge after expiration - Late payment is unavoidable $700.00 $350.00 $175.00
Surcharge after expiration - Late payment is unintentional $1,640.00 $820.00 $410.00