METHOD FOR THE IDENTIFICATION OF T CELL EPITOPES
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United States of America Patent
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Issued Date -
Apr 4, 2013
app pub date -
Apr 21, 2011
filing date -
Apr 22, 2010
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Abandoned
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Abstract
A novel method to identify relevant T-cell epitopes recognized by CD8+ or CD4− T lymphocytes is described. The method is based on the use of mRNA fragments synthesized from cDNA encoding portions of a polypeptide of interest. mRNA fragments are introduced into antigen-presenting cells to deduce an epitope's localization in a polypeptide of interest, such as a protein antigen.
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